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Cells immobilized on patterns printed in DNA by an inkjet printer

Paper ID Volume ID Publish Year Pages File Format Full-Text
7215 541 2011 7 PDF Available
Title
Cells immobilized on patterns printed in DNA by an inkjet printer
Abstract

The ability to two-dimensionally align various kinds of cells freely onto substrate would be a useful tool for analysis of cell–cell interactions. In this study, we aimed to establish a method for attaching cells to the substrate, in which the pattern is drawn by an inkjet printer. Poly-deoxyribonucleic acid (DNA) was immobilized onto the cell surface by use of DNA-conjugated poly(ethylene) glycol-phospholipid (DNA–PEG-lipid), which is the amphiphilic conjugate of PEG-lipid and single-stranded DNA. The surface of the substrate was then modified with the complementary DNA using an inkjet printer. Finally, DNA-immobilized cells were attached onto the substrate through DNA hybridization. The use of the inkjet printer enabled us to draw the DNA pattern accurately on the substrate with a resolution of a few hundred micrometers. DNA-immobilized cells could be attached precisely along the DNA pattern on the substrate. In addition, various kinds of cells could be attached simultaneously by using various sequences of DNA. Our technique is promising for analysis of cell–cell interactions and differentiation induction in stem cell research.

Keywords
Cell adhesion; DNA; Surface modification; Gold; Biocompatibility
First Page Preview
Cells immobilized on patterns printed in DNA by an inkjet printer
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 14, May 2011, Pages 3596–3602
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering