fulltext.study @t Gmail

A single-monomer derived linear-like PEI-co-PEG for siRNA delivery and silencing

Paper ID Volume ID Publish Year Pages File Format Full-Text
7221 541 2011 7 PDF Available
Title
A single-monomer derived linear-like PEI-co-PEG for siRNA delivery and silencing
Abstract

Polyethylenimines (PEIs) are commonly used as a vehicle to deliver and protect siRNA, but the strong interaction still remains to be modulated for efficient siRNA release and silencing. Herein, a single-monomer derived linear-like PEI-co-PEG (LPEI-co-PEG, P2) was synthesized to substantially enhance the siRNA release, but not affect the efficiency of protection. The linear-like copolymer (P2) was only synthesized from a single-monomer by intensive synchrotron X-ray irradiation within 5 min, randomly producing both PEI and PEG segments. The counterpart vehicle, LPEI (P1), was also synthesized for comparison. We found that the P1 and P2 were able to prevent siRNA against enzymatic degradation. Most importantly, efficient siRNA release (52%) was only observed in the siRNA/P2 complexes and not in the siRNA/P1 complexes (<5%), suggesting that the PEG segment may modulate the interaction between siRNA and P2 segment. Specifically, P2 as well as P1 can emit photoluminescence; cancer cells exhibited a detectable photoluminescence after treatment with P1 and P2, indicative of their excellent transfection efficiency. Subsequently, the siGFP/P2 complexes knocked down GFP with excellent efficiency (75%) above the siGFP/P1 complexes (19%) and siGFP/Lipofectamine complexes (20%). Importantly, the siRNA with anti-VEGF function being associated with P2 have been demonstrated an excellent efficiency in the suppression of tumor growth.

Keywords
A single-monomer copolymerization; LPEI-co-PEG; Linear-like polyethylenimine-co-poly(ethylene glycol); A non-toxic vehicle; Synchrotron X-rays irradiation; Enhanced siRNA release and mRNA silencing
First Page Preview
A single-monomer derived linear-like PEI-co-PEG for siRNA delivery and silencing
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 14, May 2011, Pages 3647–3653
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us