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Intracellular delivery of paclitaxel using oil-free, shell cross-linked HSA – Multi-armed PEG nanocapsules

Paper ID Volume ID Publish Year Pages File Format Full-Text
7255 542 2011 10 PDF Available
Title
Intracellular delivery of paclitaxel using oil-free, shell cross-linked HSA – Multi-armed PEG nanocapsules
Abstract

Various approaches to increase the solubility of water-insoluble anti-cancer drugs in aqueous formulations have been undertaken with the aim of treating solid tumors through intravenous drug administration. Nanoscale drug carriers are particularly attractive for cancer therapy because of their passive targeting effect to enhance the therapeutic efficacy of drugs. Here we introduce an oil-free, shell cross-linked nanocapsule as an efficient intracellular delivery system for paclitaxel. The nanocapsules are prepared by emulsifying amine-reactive six-arm-branched polyethylene glycol (PEG) in dichloromethane into aqueous solution of human serum albumin (HSA), followed by cross-linking at the organic/aqueous interface. Paclitaxel is successfully incorporated into the HSA/PEG nanocapsules having a spherical shape with an average diameter of about 280 nm. In several types of cells, the surface modification of nanocapsules with a cell-penetrating peptide, Hph1, greatly facilitates cellular uptake and apoptosis-inducing effects of paclitaxel. Furthermore, the targeted anti-tumor activities of the paclitaxel-loaded nanocapsules in a mouse tumor model suggest that the shell cross-linked nanocapsules are very promising oil-free nanoscale delivery vehicles for water-insoluble anti-cancer agents.

Keywords
Paclitaxel; Drug delivery system; Human serum albumin; Nanocapsule; Cell-penetrating peptide
First Page Preview
Intracellular delivery of paclitaxel using oil-free, shell cross-linked HSA – Multi-armed PEG nanocapsules
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 33, November 2011, Pages 8635–8644
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering