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Laminin active peptide/agarose matrices as multifunctional biomaterials for tissue engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
7299 544 2012 8 PDF Available
Title
Laminin active peptide/agarose matrices as multifunctional biomaterials for tissue engineering
Abstract

Cell adhesive peptides derived from extracellular matrix components are potential candidates to afford bio-adhesiveness to cell culture scaffolds for tissue engineering. Previously, we covalently conjugated bioactive laminin peptides to polysaccharides, such as chitosan and alginate, and demonstrated their advantages as biomaterials. Here, we prepared functional polysaccharide matrices by mixing laminin active peptides and agarose gel. Several laminin peptide/agarose matrices showed cell attachment activity. In particular, peptide AG73 (RKRLQVQLSIRT)/agarose matrices promoted strong cell attachment and the cell behavior depended on the stiffness of agarose matrices. Fibroblasts formed spheroid structures on the soft AG73/agarose matrices while the cells formed a monolayer with elongated morphologies on the stiff matrices. On the stiff AG73/agarose matrices, neuronal cells extended neuritic processes and endothelial cells formed capillary-like networks. In addition, salivary gland cells formed acini-like structures on the soft matrices. These results suggest that the peptide/agarose matrices are useful for both two- and three-dimensional cell culture systems as a multifunctional biomaterial for tissue engineering.

Keywords
ECM (extracellular matrix); Laminin; Peptide; Polysaccharide; Cell cultureECM, extracellular matrix; BME, basement membrane extract; FBS, fetal bovine serum; HSG, Human submandibular gland; BSA, bovine serum albumin; HDF, human dermal fibroblasts; DMEM, D
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Laminin active peptide/agarose matrices as multifunctional biomaterials for tissue engineering
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 16, June 2012, Pages 4118–4125
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us