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Array-based functional screening of growth factors toward optimizing neural stem cell microenvironments

Paper ID Volume ID Publish Year Pages File Format Full-Text
7338 550 2011 8 PDF Available
Title
Array-based functional screening of growth factors toward optimizing neural stem cell microenvironments
Abstract

To gain insights into the effect of various growth factors on the behaviors of neural stem cells, cell culture assays were performed on the array that displayed five different growth factors including basic fibroblast growth factor, epidermal growth factor, insulin-like growth factor-1, brain-derived neurotrophic factor, and ciliary neurotrophic factor. These factors were expressed in Escherichia coli as fusion proteins with a hexahistidine sequence and arrayed on a nickel ion-functionalized chip as single factors or the combination of two factors. Neural stem cells obtained from the fetal rat brain were cultured on the array to investigate their proliferation and differentiation. It was shown that the five growth factors displayed as a single component had significant impacts on cell behaviors. These effects are overall in accordance with those reported previously. On the other hand, in the case that two different growth factors were co-displayed on a single spot, the behaviors of neural stem cells could not be simply predicted from their individual effects. We performed a multivariate cluster analysis for the quantitative data on cell proliferation and differentiation. It was shown that the effect of two growth factors co-displayed was competitive, synergistic, or destructive depending on the combinations. In other peculiar cases, the effect of growth factors was totally different from those of individual factors.

Keywords
Cell proliferation; Growth factors; Micropatterning; Neural cell; Recombinant protein; Stem cell
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Array-based functional screening of growth factors toward optimizing neural stem cell microenvironments
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 22, August 2011, Pages 5015–5022
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us