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Tumor transfection after systemic injection of DNA lipid nanocapsules

Paper ID Volume ID Publish Year Pages File Format Full-Text
7420 552 2011 7 PDF Available
Title
Tumor transfection after systemic injection of DNA lipid nanocapsules
Abstract

With the goal of generating an efficient vector for systemic gene delivery, a new kind of nanocarrier consisting of lipid nanocapsules encapsulating DOTAP/DOPE lipoplexes (DNA LNCs) was pegylated by the post-insertion of amphiphilic and flexible polymers. The aim of this surface modification was to create a long-circulating vector, able to circulate in the blood stream and efficient in transfecting tumoral cells after passive targeting by enhanced permeability and retention effect (EPR effect). PEG conformation, electrostatic features, and hydrophylicity are known to be important factors able to influence the pharmacokinetic behaviour of vectors. In this context, the surface structure characteristics of the newly pegylated DNA LNCs were studied by measuring electrophoretic mobility as a function of ionic strength in order to establish a correlation between surface properties and in vivo performance of the vector. Finally, thanks to this PEGylation, gene expression was measured up to 84-fold higher in tumor compared to other tested organs after intravenous injection. The present results indicate that PEGylated DNA LNCs are promising carriers for an efficient cancer gene therapy.

Keywords
Intravenous injection; Gene delivery; Non-viral vector; Pegylation-nanoparticles
First Page Preview
Tumor transfection after systemic injection of DNA lipid nanocapsules
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 9, March 2011, Pages 2327–2333
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering