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Interactions of vimentin- or desmin-expressing liver cells with N-acetylglucosamine-bearing polymers

Paper ID Volume ID Publish Year Pages File Format Full-Text
7455 553 2012 11 PDF Available
Title
Interactions of vimentin- or desmin-expressing liver cells with N-acetylglucosamine-bearing polymers
Abstract

It is necessary to develop highly functionalized liver cell culture systems for liver tissue engineering such as bioartificial livers and liver cell chips. To maintain a high level of hepatocyte function, well-organized patterning culture systems of hepatocytes and nonparenchymal cells would be advantageous. To design the patterning culture system using these cells, cell-recognizable polymers should be useful to regulate not only the hepatocytes, but also the nonparenchymal cells. Here, we report that N-acetylglucosamine (GlcNAc)-bearing polymers are useful as nonparenchymal cell-recognizable polymers. It has previously been reported that mesenchymal cells adhered to GlcNAc-bearing polymer-coated dishes through surface vimentin. It was also observed that nonparenchymal cells expressing vimentin or desmin specifically adhered to GlcNAc-bearing polymer-coated dishes. Especially, in hepatic stellate cells (HSCs) cultured on GlcNAc-bearing polymer-coated dishes, the expression of α-smooth muscle actin as an activated HSCs marker was suppressed in long-term. Therefore, HSCs were shown to maintain a quiescent state on PVGlcNAc-coated dishes during a long-term culture. These results demonstrated that GlcNAc-bearing polymers could be beneficial to culture nonparenchymal cells such as HSCs. Our findings suggest that galactose- and GlcNAc-bearing polymers can regulate the culture of all liver cells and may be useful tools for the establishment of liver tissue engineering.

Keywords
Cell adhesion; Hepatocyte; Liver tissue engineering; Nonparenchymal cells; Hepatic stellate cells
First Page Preview
Interactions of vimentin- or desmin-expressing liver cells with N-acetylglucosamine-bearing polymers
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 7, March 2012, Pages 2154–2164
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering