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Size-dependent transfection efficiency of PEI-coated gold nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
746 61 2011 11 PDF Available
Title
Size-dependent transfection efficiency of PEI-coated gold nanoparticles
Abstract

Gold nanoparticles (Au NPs) are promising vectors for gene delivery applications. In order to gain insight on the influence of particle size on cell transfection, Au NPs were combined with poly(ethylenimine) (PEI) to prepare two sets of PEI-coated Au NPs having particle-size distributions centered at about 6 nm (<10 nm Au-PEI NPs) or 70 nm (<100 nm Au-PEI NPs), respectively. Au-PEI NPs were coupled to a variety of plasmids carrying reporter or suicide genes to prepare Au-PEI NPs/DNA complexes, and human osteosarcoma Saos-2 cells were used to investigate the performance of the Au-PEI NPs as transfection vectors in serum-containing media. The conjugates of DNA with both types of Au-PEI NPs were found to be negatively charged. In spite of the electrostatic repulsion that occurs between the surface of the cell and the surface of the plasmid-conjugated NPs, cell internalization was observed for both kinds of Au-PEI NPs. Cells were efficiently transfected with complexes derived from <10 nm Au-PEI NPs, but not with the <100 nm Au-PEI NPs. Large aggregates of NPs associated with DNA were found in endocytic vesicles of cells incubated with <100 nm Au-PEI NPs, while the success of the smaller Au-PEI NPs as transfection vectors was related to their lower agglomeration state inside cells and to endosomal escape of DNA.

Keywords
Nanoparticles; Transfection; Cell trafficking; Transgene delivery; Gold
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Size-dependent transfection efficiency of PEI-coated gold nanoparticles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 10, October 2011, Pages 3645–3655
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us