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Engineering of living autologous human umbilical cord cell-based septal occluder membranes using composite PGA-P4HB matrices

Paper ID Volume ID Publish Year Pages File Format Full-Text
7490 554 2011 12 PDF Available
Title
Engineering of living autologous human umbilical cord cell-based septal occluder membranes using composite PGA-P4HB matrices
Abstract

Interventional closure of intracardiac wall defects using occluder devices has evolved as a highly attractive treatment option. However, incomplete and delayed healing reactions often result in a major risk of residual defects, thromboembolism, or device fractures. Biodegradable living tissue engineered occluder membranes (TEOMs) could provide autologous thromboresistant implants with growth and remodeling capacities. PGA-P4HB composite matrices were seeded with human umbilical cord-derived cells or vascular-derived control cells and exposed to static (n = 19) or dynamic (n = 13) conditioning. Harvested TEOMs were integrated into occluder frameworks, exposed to crimping and delivered into pre-formed defects of juvenile porcine hearts. Dynamically conditioned TEOM constructs showed higher collagen formation in histology than static constructs with significantly higher stiffness moduli in uniaxial tensile testing. Grating interferometry revealed substantial but inhomogeneous cone-like degradation of the composite matrices in dynamic conditioning. The crimping and delivery procedures resulted in no significant changes in macroscopy, histo-morphology, cellular viability, DNA or hydroxyproline content, and scanning electron microscopy findings. Here, we present the in vitro fabrication, crimping and experimental delivery of living human umbilical cord-cell derived TEOMs based on composite matrices as a potential future autologous therapy of intracardiac wall defects.

Keywords
Umbilical cord cells; Biodegradable matrix; Septal occluder system; Polyglycolic acid; Poly-4-hydroxybutyrate; Crimping
First Page Preview
Engineering of living autologous human umbilical cord cell-based septal occluder membranes using composite PGA-P4HB matrices
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 36, December 2011, Pages 9630–9641
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering