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The effect of micro and nanotopography on endocytosis in drug and gene delivery systems

Paper ID Volume ID Publish Year Pages File Format Full-Text
7514 554 2011 10 PDF Available
Title
The effect of micro and nanotopography on endocytosis in drug and gene delivery systems
Abstract

Endocytosis is a fundamental biological process and is also the key mechanism for drug and non-viral gene delivery. The importance of topographical cues in modulating cell behaviors has become increasingly evident, but the influence of topography on endocytosis has however only been sparsely studied. We hypothesize that topography can enhance cellular endocytosis, and in turn the non-viral transfection efficiency. Nano- to microtopographical patterns were fabricated using nano-imprinting lithography (NIL). We first investigated if the substrate topographies could modulate endocytosis and in turn the cellular transfectability. Our results showed increased internalization of fluorescently labeled dextran by human mesenchymal stem cells (hMSCs) and monkey kidney fibroblasts (COS7) when they were cultured on micro- and nanopillars. When the hMSCs were introduced to green-fluorescent protein (GFP) encoding plasmid with Lipofectamine, highest transfection efficiency was observed in cells on nanopillars. Tunable detachable substrate topographies were also fabricated using NIL to promote endocytosis in different cell types, and our results show hMSCs phagocytosis of these polymeric structures. Besides being important in understanding the fundamental process of endocytosis, the current research results may also lead to applications utilizing nanotopography to enhance drug and gene delivery.

Keywords
Topography; Nanoimprinting; Non-viral transfection; Human mesenchymal stem cells; Phagocytosis; Transfection efficiency
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The effect of micro and nanotopography on endocytosis in drug and gene delivery systems
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 36, December 2011, Pages 9866–9875
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us