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Immunostimulatory activity of polysaccharide–poly(I:C) nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
7534 555 2011 8 PDF Available
Title
Immunostimulatory activity of polysaccharide–poly(I:C) nanoparticles
Abstract

Immunostimulatory properties of mushroom derived polysaccharides (PS) as stand-alone agents were tested. Next, PS were nanocomplexed with polyI:C (pIC) to yield stable nanoparticles around 200 nm in size evidenced by atomic force microscopy and dynamic light scattering analyses. PSs were selectively engaged by cells expressing TLR2 and initiated NFκB dependent signaling cascade leading to a Th1-biased cytokine/chemokine secretion in addition to bactericidal nitric oxide (NO) production from macrophages. Moreover, cells treated with nanoparticles led to synergistic IL6, production and upregulation of TNFα, MIP3α, IFNγ and IP10 transcript expression. In mice, PS-Ovalbumin-pIC formulation surpassed anti-OVA IgG responses when compared to either PS-OVA or pIC-OVA mediated immunity. Our results revealed that signal transduction initiated both by TLR2 and TLR3 via co-delivery of pIC by PS in nanoparticle depot delivery system is an effective immunization strategy. The present work implicate that the PS and nucleic acid based nanoparticle approach along with protein antigens can be harnessed to prevent infectious diseases.

Keywords
Polysaccharides; Poly(I:C); Nanoparticles; Targeted delivery system; TLR; ImmunogenicityAFM, Atomic Force Microscopy; BMDC, Bone marrow derived dendritic cell; HEK, Human embryonic kidney; NOD, Nucleotide-binding oligomerization domain; ODN, Oligodeoxynuc
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Immunostimulatory activity of polysaccharide–poly(I:C) nanoparticles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 18, June 2011, Pages 4275–4282
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
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Full-text PDF Download
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