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The use of multifunctional magnetic mesoporous core/shell heteronanostructures in a biomolecule separation system

Paper ID Volume ID Publish Year Pages File Format Full-Text
7539 556 2011 8 PDF Available
Title
The use of multifunctional magnetic mesoporous core/shell heteronanostructures in a biomolecule separation system
Abstract

A multifunctional magnetic mesoporous core/shell heteronanostructure (designated as Fe3O4@NiSiO3) has been designed and constructed that combined the capacity of effective protein purification from protein mixture and selective low molecule weight (MW) biomolecule enrichment. The nanoparticle is composed by magnetite nanoparticle with immobilized metal ion surface and solid porous shell which presents a number of important features, such as controllable shell thickness, uniform pore size and excellent magnetism. By taking advantages of the high affinity of Ni2+ on the shell surface toward His-tagged proteins and the fast response toward an assistant magnet, the heteronanoparticles can be applied to selectively bind to and magnetically separate of His-tagged proteins from a cell lysate of E. coli. Additionally, owing to the homogeneous 3D mesopores on the nickel silicate shell, the heteronanoparticles can selectively capture low MW biomolecules from complex mixture. Significantly, it is expected that this approach can be extended to other biomolecule separation and enrichment systems by changing the immobilized surface and the pore size.

Keywords
Heteronanostructure; Mesoporous material; Protein separation; Peptide enrichment; Bionanotechnology
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 21, July 2011, Pages 4683–4690
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us