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The effect of coimmobilizing heparin and fibronectin on titanium on hemocompatibility and endothelialization

Paper ID Volume ID Publish Year Pages File Format Full-Text
7540 556 2011 13 PDF Available
Title
The effect of coimmobilizing heparin and fibronectin on titanium on hemocompatibility and endothelialization
Abstract

Currently available cardiovascular implants, such as heart valves and stents, exhibit suboptimal biocompatibility because of the incomplete endothelialization and sequential thrombosis formation especially after a long-term implantation. To improve the blood compatibility and endothelialization simultaneously and ensure the long-term effect of the cardiovascular implants, a technique of combining electrostatic interaction and coimmobilization was developed to form heparin and fibronectin (Hep/Fn) films on aminosilanized titanium (Ti) surfaces. The Hep/Fn coimmobilized films were stable after immersion in PBS for five days, probed by wettability studies and by the release kinetics of heparin and fibronectin. Blood compatibility tests showed that the coimmobilized Hep/Fn films displayed lower hemolysis rate, prolonged blood coagulation time, higher AT III binding density, less platelets activation and aggregation, and less fibrinogen conformational change compared with Ti surface. Endothelial cells (ECs) seeding and fibronectin bioactivity results showed more attached and proliferated ECs and exposed cell-binding sites on the Hep/Fn immobilized samples than that on Ti surfaces. Thus, the Hep/Fn coimmobilized films kept excellent bioactivity even after immersion in PBS for five days. Systemic evaluation suggests that the coimmobilization of Hep/Fn complex improves the blood compatibility and promotes the endothelialization simultaneously. We envisage that this method will provide a potential and effective selection for biomaterials surface modification of cardiovascular implants.

Keywords
Coimmobilization; Heparin; Fibronectin; Stability; Blood compatibility; Endothelialization
First Page Preview
The effect of coimmobilizing heparin and fibronectin on titanium on hemocompatibility and endothelialization
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 21, July 2011, Pages 4691–4703
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering