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Chemical surface modification of parylene C for enhanced protein immobilization and cell proliferation

Paper ID Volume ID Publish Year Pages File Format Full-Text
756 61 2011 11 PDF Available
Title
Chemical surface modification of parylene C for enhanced protein immobilization and cell proliferation
Abstract

To introduce the adhesion site of proteins and/or cells on parylene C (PC)-coated medical devices that can be used as implantable biosensors or drug delivery capsules, the PC surfaces were initially modified by the Friedel–Crafts acylation reaction to generate active chlorines. These chlorines were then employed to initiate the atom transfer radical polymerization of tert-butyl acrylate (TBA) and form a polymer brush layer of polyTBA on PC; the acrylate groups in the polymer brushes were hydrolyzed to carboxylic acid groups and further activated into succinimidyl ester groups via the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide coupling reaction. The PC surface grafted with polymer brushes and activated by succinimide showed efficient attachment of proteins, including gelatin, contortrostatin (CN) and bovine serum albumin (BSA), all at high density on the PC surface. The CN density on the surface was evaluated for both monolayer and polymer brush-based coatings. Based on fluorescence measurements, the polymer brush gives a 60-fold higher surface protein density than the monolayer-based system. Gelatin was used as a model protein and covalently coated onto the modified PC surface for cell culture study. Substrates with gelatin coating showed a significantly higher cell attachment and proliferation in 7 days cultures as compared to the uncoated substrates. In addition, a conventional photolithography technique was coupled with the surface chemistry to successfully pattern the BSA labeled with fluorescein isothiocyanate on the modified PC surfaces.

► Parylene C (PC) was surface modified by chemical reactions to immobilize the functional polymer brushes or monolayer groups for protein immobilization. ► The polymer brushes based PC surface exhibited the significantly higher amount of protein attachment than that of the monolayer based PC surface. ► Polymer brushes based PC was coated with gelatin, and cell showed higher attachment and proliferation than that on monolayer based PC. ► Photolithography technique was applied to pattern the protein onto modified PC.

Keywords
Parylene C; Surface modification; Polymer brushes; Protein; Cell culture
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Chemical surface modification of parylene C for enhanced protein immobilization and cell proliferation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 10, October 2011, Pages 3746–3756
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us