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Differential affinity of vitronectin versus collagen for synthetic biodegradable scaffolds for urethroplastic applications

Paper ID Volume ID Publish Year Pages File Format Full-Text
7585 557 2011 11 PDF Available
Title
Differential affinity of vitronectin versus collagen for synthetic biodegradable scaffolds for urethroplastic applications
Abstract

Cell-seeded synthetic polymer scaffolds constitute an emerging technology for urethroplastic applications. The study goal was to identify urethral proteins appropriate for cell attachment and optimize their adsorption onto two types of scaffolds: porous poly(ester urethane) with a poly(caprolactone) soft segment (PEU-PCL) and poly-(96% L/4% D)-lactic acid (P96L/4DLA). Specimens from eight men undergoing urethral reconstruction for stricture diseases were subjected to immunohistochemical analysis. Type I collagen, type IV collagen and vitronectin were detected at the interface between the epithelium and its basement membrane. Electrophoresis confirmed that polypeptide chains in the starting material were also present in fractions eluted from adsorbed scaffolds. Over a 4 week incubation assay, only vitronectin exhibited 100% retention levels for all scaffolds. The saturation point for each protein on each scaffold type was determined by titration and ELISA. The collective evidence indicates the concept that vitronectin > type IV collagen > type I collagen are preferred adsorption proteins for PEU-PCL and P96L/4DLA.

Keywords
Porous polyurethane; Poly-96L/4D-lactide; Type I collagen; Type IV collagen; Vitronectin; Saturation
First Page Preview
Differential affinity of vitronectin versus collagen for synthetic biodegradable scaffolds for urethroplastic applications
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 3, January 2011, Pages 797–807
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering