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Fibronectin layers by matrix-assisted pulsed laser evaporation from saline buffer-based cryogenic targets

Paper ID Volume ID Publish Year Pages File Format Full-Text
760 61 2011 9 PDF Available
Title
Fibronectin layers by matrix-assisted pulsed laser evaporation from saline buffer-based cryogenic targets
Abstract

The deposition of fibronectin (FN) from saline buffer-based cryogenic targets by matrix-assisted pulsed laser evaporation (MAPLE) onto silicon substrates is reported. A uniform distribution of FN was revealed by Ponceau staining after control experiments on nitrocellulose paper. Well-organized particulates with heights from hundreds of nanometers up to more than 1 μm packed in homogeneous layers were evidenced by optical microscopy and profilometry on Si substrates. Atomic force microscopy images showed regions composed of buffer and FN aggregates forming a compact film. Comparison of infrared spectra of drop-cast and MAPLE-deposited FN confirmed the preservation of composition and showed no degradation of the protein. The protein deposition on Si was confirmed by antibody staining. Small aggregates and fluorescent fibrils were visualized by fluorescence microscopy. Superior attachment of human osteoprogenitor cells cultivated for 3 h proved the presence of stable and intact FN molecules after transfer.

Keywords
Fibronectin layers; MAPLE transfer; FTIR; Immunostaining; Osteoprogenitor cell attachment
First Page Preview
Fibronectin layers by matrix-assisted pulsed laser evaporation from saline buffer-based cryogenic targets
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 10, October 2011, Pages 3780–3788
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering