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Molecular mechanisms of antibacterial and antitumor actions of designed surfactant-like peptides

Paper ID Volume ID Publish Year Pages File Format Full-Text
7624 558 2012 12 PDF Available
Title
Molecular mechanisms of antibacterial and antitumor actions of designed surfactant-like peptides
Abstract

Biomimicry of antimicrobial peptides secreted by innate immune systems represents a major strategy in developing novel antibacterial treatments. There are however emerging concerns over the possible compromise of host natural defenses by these biomimetic peptides due to their structural similarity. In our recent work we have extended the search by exploring the potential from unnatural synthetic antimicrobial peptides. Here we show that a series of surfactant-like peptides (AmKn, m ≥ 3, n = 1, 2) can kill not only bacteria but also cancerous HeLa cells in similar manner. Under the same experimental conditions, however, these peptides showed little affinity to NIH 3T3 cells and human red blood cells (hRBCs), thus demonstrating high biocompatibility in selective responses to host mammalian cells and low hemolysis. A9K1 was most effective in killing HeLa cells, a trend consistent with their bactericidal effects against Escherichia coli and Bacillus subtilis. Mechanistic investigations through combined studies of SEM and fluorescence assays revealed that the killing of bacteria and cancerous cells was caused by disrupting cell membranes, initiated by electrostatic interactions between cationic peptides and negatively charged cell membranes. In contrast, the absence of such interactions in the case of NIH 3T3 and hRBCs over the same peptide concentration range rendered low cytotoxicity. The most effective killing power of A9K1 within this series benefited from the combined effects of several factors including modest micellar concentration and balanced amphiphilicity, consistent with its propensity of self-assembly and effective membrane lytic power.

Keywords
Antimicrobial peptides; Surfactant-like peptides; Peptide self assembly; Cell selectivity; Membrane permeability
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Molecular mechanisms of antibacterial and antitumor actions of designed surfactant-like peptides
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 2, January 2012, Pages 592–603
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us