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Construction of the recellularized corneal stroma using porous acellular corneal scaffold

Paper ID Volume ID Publish Year Pages File Format Full-Text
7647 559 2011 10 PDF Available
Title
Construction of the recellularized corneal stroma using porous acellular corneal scaffold
Abstract

Acellular porcine cornea stroma (APCS) prepared using pancreatic phospholipase A2 was proven to be promising corneal scaffold. However, its dense ultrastructure provides insufficient space that prevents the seeded cells from organizing into a functional tissue. In this report, freezing dry APCS (FD-APCS) biomaterials containing pores with different sizes were fabricated at different pre-freezing temperatures of −10, −80 or −198 °C, and the percentage of large pores (equivalent circle diameter ≥ 10 μm) was 93.55%, 69.36%, 35.79%, while the small pores (<10 μm) were account for 6.45%, 30.64%, 64.21%, respectively. Both porosity and specific surface area increased in FD-APCS fabricated with decreased pre-freezing temperature, and they were dramatically higher than those in APCS. The three FD-APCS groups displayed higher permeability than APCS, and the −10 °C FD-APCS possessed the highest permeability. The keratocytes seeded in the FD-APCS construct survived well in vitro, and maximal cell proliferation was observed in the −10 °C FD-APCS. The light transmittance of the FD-APCS-transplanted cornea after interlamellar keratoplasty in rabbit eyes displayed no significant difference from the APCS-transplanted or native cornea. This study indicated that the porous FD-APCS prepared using pancreatic phospholipase A2 is capable of serving as potential scaffold for constructing tissue-engineered cornea with biological properties.

Keywords
Corneal tissue engineering; Decellularization; Scaffold; Freezing dry; Porosity
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Construction of the recellularized corneal stroma using porous acellular corneal scaffold
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 29, October 2011, Pages 6962–6971
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us