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Cellular uptake, cytotoxicity, and ROS generation with silica/conducting polymer core/shell nanospheres

Paper ID Volume ID Publish Year Pages File Format Full-Text
7673 559 2011 9 PDF Available
Title
Cellular uptake, cytotoxicity, and ROS generation with silica/conducting polymer core/shell nanospheres
Abstract

The cellular response to conducting polymer (CP) nanospheres with similar physical properties was evaluated by in vitro cellular uptake and cytotoxicity in mouse macrophage RAW 264.7 and rat pheochromocytoma PC-12 cells. Four different CPs (polythiophene, poly(3,4-ethylenedioxythiophene), polyaniline, and polypyrrole) were deposited onto silica nanoparticles with a diameter of ca. 22 nm. Cellular uptake of silica/CP core/shell nanospheres in both cell lines was observed by transmission electron microscopy and they were internalized via phagocytosis and endocytosis. Cytotoxic effects were systemically assessed using live-cell microscopy, viability, oxidative stress, and lactate dehydrogenase assays. Silica/polythiophene core/shell nanospheres were the most toxic in both cell lines examined, because of the cellular effects of sulfur atoms. On the other hand, silica/polypyrrole core/shell nanospheres caused the lowest levels of toxicity in both cell lines. Furthermore, both rat and mouse cell viability was concentration-dependent with the nanospheres. These findings enhance nanotoxicological information regarding CP nanospheres when used with macrophage and neuronal cells, which may be useful in their application in bioelectronic and biomedical fields.

► The cellular response to silica/conducting polymer core/shell nanospheres was investigated using macrophage and rat pheochromocytoma cells. ► Four different conducting polymers including polythiophene, poly(3,4-ethylenedioxythiophene), polyaniline, and polypyrrole were used as shell materials. ► Among the four types of nanospheres, silica/polythiophene core/shell nanospheres represented the highest toxicity in both cells mainly due to cellular effect of sulfur atoms.

Keywords
Conducting polymer; Core shell nanosphere; Cytotoxicity; Cell viability; Reactive oxygen species
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Cellular uptake, cytotoxicity, and ROS generation with silica/conducting polymer core/shell nanospheres
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 29, October 2011, Pages 7217–7225
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us