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One-dimensional self-assembly of mouse embryonic stem cells using an array of hydrogel microstrands

Paper ID Volume ID Publish Year Pages File Format Full-Text
7682 560 2011 8 PDF Available
Title
One-dimensional self-assembly of mouse embryonic stem cells using an array of hydrogel microstrands
Abstract

The ability of embryonic stem (ES) cells to self-renew indefinitely and to differentiate into multiple cell lineages holds promise for advances in modeling disease progression, screening drugs and treating diseases. To realize these potentials, it is imperative to study self-assembly in an embryonic microenvironment, as this may increase our understanding of ES cell maintenance and differentiation. In this study, we synthesized an array of one-dimensional alginate gel microstrands and aqueous microstrands through an SU-8 filter device by means of capillary action. Furthermore, we investigated self-assembly behaviors and differentiation potentials of mouse ES cells cultured in microstrands of varying diameters. We found that microstrands with an aqueous interior facilitated high density cell culture and formed compact microtissue structures, while microstrands with gelled interiors promote smaller cell aggregate structures. In particular, we noticed that ES cells collected from one-dimensional aqueous microstrands favored the differentiation towards cell lineages of endoderm and mesoderm, whereas those from gelled microstrands preferred to differentiate into ectoderm and mesoderm lineages. In addition to providing a “liquid-like” tubular microenvironment to understand one-dimensional self-assembly process of ES cells, this alginate hydrogel microstrand system also offers an alternative way to manipulate the stem cell fate-decision using bioengineered microenvironments.

Keywords
Stem cell; Alginate; Hydrogel; Self-assembly; Microfiber; Microfluidics
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One-dimensional self-assembly of mouse embryonic stem cells using an array of hydrogel microstrands
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 20, July 2011, Pages 4498–4505
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us