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Targeted dual-contrast T1- and T2-weighted magnetic resonance imaging of tumors using multifunctional gadolinium-labeled superparamagnetic iron oxide nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
7691 560 2011 10 PDF Available
Title
Targeted dual-contrast T1- and T2-weighted magnetic resonance imaging of tumors using multifunctional gadolinium-labeled superparamagnetic iron oxide nanoparticles
Abstract

Development of a multifunctional nanoparticle (NP) system allowing for dual-contrast T1- and T2-weighted targeted magnetic resonance (MR) imaging of tumors could significantly improve the diagnosis accuracy. In this study, superparamagnetic silica-coated iron oxide core-shell nanoparticles (Fe3O4@SiO2 NPs) with a diameter of approximately 21 nm were synthesized via a thermal decomposition approach and were aminated through silanization. The amine-functionalized Fe3O4@SiO2 NPs enabled the covalent conjugation of a paramagnetic gadolinium complex (Gd-DTPA, DTPA: diethylenetriamine pentaacetic acid) and an arginine-glycine-aspartic acid (RGD) peptide as a targeting ligand onto their surface. The formed Fe3O4@SiO2(Gd-DTPA)-RGD NPs are water-dispersible, stable, and biocompatible as confirmed by MTT cell viability assay. Relaxivity measurements show that they have a T1 relaxivity (r1) of 4.2 mm−1 s−1 and T2 relaxivity (r2) of 17.4 mm−1 s−1 at the Gd/Fe molar ratio of 0.3:1, suggesting a possibility to use them as both T1 positive and T2 negative contrast agents. In vitro and in vivo MR imaging experiments show that the developed multifunctional Fe3O4@SiO2(Gd-DTPA)-RGD NPs enable targeted dual-contrast T1- and T2-weighted MR imaging of tumor cells over-expressing high-affinity αvβ3 integrin in vitro and in vivo. Our results clearly indicate that the approach to forming multifunctional Fe3O4@SiO2(Gd-DTPA)-RGD NPs could be extended for fabricating other biologically active NPs for T1- and T2-weighted MR imaging of other biological systems with high accuracy.

Keywords
Dual-contrast; Targeted; Tumors; Magnetic resonance imaging; Gadolinium-labeled; Superparamagnetic iron oxide
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Targeted dual-contrast T1- and T2-weighted magnetic resonance imaging of tumors using multifunctional gadolinium-labeled superparamagnetic iron oxide nanoparticles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 20, July 2011, Pages 4584–4593
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us