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Engineering membrane scaffolds with both physical and biomolecular signaling

Paper ID Volume ID Publish Year Pages File Format Full-Text
770 63 2012 12 PDF Available
Title
Engineering membrane scaffolds with both physical and biomolecular signaling
Abstract

We report on the combination of a top-down and bottom-up approach to develop thin bioactive membrane scaffolds based on functional elastin-like polymers (ELPs). Our strategy combines ELP cross-linking and assembly, and a variety of standard and novel micro/nanofabrication techniques to create self-supporting membranes down to ∼500 nm thick that incorporate both physical and biomolecular signals, which can be easily tailored for a specific application. In this study we used an ELP that included the cell-binding motif arginine–glycine–aspartic acid–serine (RGDS). Furthermore, fabrication processes were developed to create membranes that exhibited topographical patterns with features down to 200 nm in lateral dimensions and up to 10 μm in height on either one or both sides, uniform and well-defined pores, or multiple ELP layers. A variety of processing parameters were tested in order to optimize membrane fabrication, including ELP and cross-linker concentration, temperature, reaction time and ambient humidity. Membrane micro/nanopatterning, swelling and stiffness were characterized by atomic force microscopy, nanoindentation tests and scanning electron microscopy. Upon immersion in phosphate-buffered saline and an increase in temperature from 25 to 40 °C, membranes exhibited a significant increase in surface stiffness, with the reduced Young’s modulus increasing with temperature. Finally, rat mesenchymal stem cells were cultured on thin RGDS-containing membranes, which allowed cell adhesion, qualitatively enhanced spreading compared to membranes without RGDS epitopes and permitted proliferation. Furthermore, cell morphology was drastically affected by topographical patterns on the surface of the membranes.

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Keywords
Membranes; Scaffolds; Tissue engineering; Nanotechnology; Elastin-like polymers
First Page Preview
Engineering membrane scaffolds with both physical and biomolecular signaling
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 8, Issue 3, March 2012, Pages 998–1009
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering