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The performance of laminin-containing cryogel scaffolds in neural tissue regeneration

Paper ID Volume ID Publish Year Pages File Format Full-Text
7710 561 2011 12 PDF Available
Title
The performance of laminin-containing cryogel scaffolds in neural tissue regeneration
Abstract

Currently, there are no effective therapies to restore lost brain neurons, although rapid progress in stem cell biology and biomaterials development provides new tools for regeneration of central nervous system. Here we describe neurogenic properties of bioactive scaffolds generated by cryogelation of dextran or gelatin linked to laminin – the main component of brain extracellular matrix. We showed that such scaffolds promoted differentiation of human cord blood-derived stem cells into artificial neural tissue in vitro. Our experiments revealed that optimal range of scaffolds’ pore size for neural tissue engineering was 80–100 microns. We found that scaffold seeded with undifferentiated, but not neutrally committed stem cells, gave optimal cell adhesion and proliferation in “niche”-like structures. Subsequent differentiation resulted in generation of mature 3D networks of neurons (MAP2+) and glia (S100beta+) cells. We showed that cryogel scaffolds could be transplanted into the brain tissue or organotypic hippocampal slices in a rat models. The scaffolds did not induced inflammation mediated by microglial cells (ED1-, Ox43-, Iba1-) and prevented formation of glial scar (GFAP-). Contrary, laminin-rich scaffolds attracted infiltration of host’s neuroblasts (NF200+, Nestin+) indicating high neuroregeneration properties.

Keywords
Bioactivity; Biocompatibility; Transplantation; Cross-linking; Nerve tissue engineering; Stem cell
First Page Preview
The performance of laminin-containing cryogel scaffolds in neural tissue regeneration
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 13, May 2011, Pages 3423–3434
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering