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Cell–Cell interactions of human neural progenitor-derived astrocytes within a microstructured 3D-scaffold

Paper ID Volume ID Publish Year Pages File Format Full-Text
7733 562 2010 11 PDF Available
Title
Cell–Cell interactions of human neural progenitor-derived astrocytes within a microstructured 3D-scaffold
Abstract

In the present in vitro study, the axon growth promoting effects of human neural progenitor-derived astrocytes (hNP-AC) were investigated in simple 2D- as well as in more complex 3D-culture systems. The interactions of the hNP-AC with migrating Schwann cells and fibroblasts were also studied. hNP-AC were found to promote extensive dorsal root ganglion axon regeneration in 2D cultures, being even greater than that observed on the positive control, laminin-coated substrate. Contact-mediated mechanisms and the release of substances into the medium both played a role in supporting axon regeneration. Following seeding onto 3D collagen scaffolds, hNP-AC also promoted significantly greater axon regeneration from DRG explants than was seen on non-seeded scaffolds. The highly orientated, porous microstructure of the scaffold also supported substantial intermixing of hNP-AC and migrating Schwann cells/fibroblasts from the DRG explant, cell populations that are normally mutually repulsive. This suggests that the topography of 3D scaffolds may not only influence cell–substrate interactions but also cell–cell interactions within the scaffold. This opens the possibility that the design of future scaffolds could be optimised to enhance cell integration as well as modulating complex cell–cell interactions.

Keywords
Neural progenitor cells; Astrocytes; Collagen; Three dimensional; Nerve regeneration; Cell–cell interaction
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Cell–Cell interactions of human neural progenitor-derived astrocytes within a microstructured 3D-scaffold
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 30, October 2010, Pages 7705–7715
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us