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Damage associated molecular patterns within xenogeneic biologic scaffolds and their effects on host remodeling

Paper ID Volume ID Publish Year Pages File Format Full-Text
7757 563 2012 11 PDF Available
Title
Damage associated molecular patterns within xenogeneic biologic scaffolds and their effects on host remodeling
Abstract

The immune response is an important determinant of the downstream remodeling of xenogeneic biologic scaffolds in vivo. Pro-inflammatory responses have been correlated with encapsulation and a foreign body reaction, while anti-inflammatory reactions are associated with constructive remodeling. However, the bioactive and bioinductive molecules within the extracellular matrix (ECM) that induce this polarization are unclear, although it is likely that cellular remnants such as damage associated molecular patterns (DAMPs) retained within the scaffold may play a role. The present study investigated the immunomodulatory effects of common ECM scaffolds. Results showed that tissue source, decellularization method and chemical crosslinking modifications affect the presence of the well characterized DAMP - HMGB1. In addition, these factors were correlated with differences in cell proliferation, death, secretion of the chemokines CCL2 and CCL4, and up regulation of the pro-inflammatory signaling receptor toll-like receptor 4 (TLR4). Inhibition of HMGB1 with glycyrrhizin increased the pro-inflammatory response, increasing cell death and up regulating chemokine and TLR4 mRNA expression. The present study suggests the importance of HMGB1 and other DAMPS as bioinductive molecules within the ECM scaffold. Identification and evaluation of other ECM bioactive molecules will be an area of future interest for new biomaterial development.

Keywords
Extracellular matrix; Immune response; Immunomodulation; MonocyteDAMP, damage associated molecular patterns; ECM, extracellular matrix; HA, hyaluronic acid; HMGB1, high mobility group box 1; HSG, heparin sulfate proteoglycan; HSP70, heat shock protein 70;
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Damage associated molecular patterns within xenogeneic biologic scaffolds and their effects on host remodeling
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 1, January 2012, Pages 91–101
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us