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An FRET-based ratiometric chemosensor for in vitro cellular fluorescence analyses of pH

Paper ID Volume ID Publish Year Pages File Format Full-Text
7765 563 2012 10 PDF Available
Title
An FRET-based ratiometric chemosensor for in vitro cellular fluorescence analyses of pH
Abstract

Ratiometric fluorescence sensing is an important technique for precise and quantitative analysis of biological events occurring under complex conditions by simultaneously recording fluorescence intensities at two wavelengths and calculating their ratios. Herein, we design a ratiometric chemosensor for pH that is based on photo-induced electron transfer (PET) and binding-induced modulation of fluorescence resonance energy transfer (FRET) mechanisms. This ratiometric chemosensor was constructed by introduction of a pH-insensitive coumarin fluorophore as an FRET donor into a pH-sensitive amino-naphthalimide derivative as the FRET acceptor. The sensor exhibited clear dual-mission signal changes in blue and green spectral windows upon pH changes. The pH sensor was applied for not only measuring cellular pH, but also for visualizing stimulus-responsive changes of intracellular pH values.

Keywords
pH sensor; Ratiometric sensing; FRET; PET; Biological imaging
First Page Preview
An FRET-based ratiometric chemosensor for in vitro cellular fluorescence analyses of pH
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 1, January 2012, Pages 171–180
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering