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The phagocytosis of gas-filled microbubbles by human and murine antigen-presenting cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
7780 563 2012 10 PDF Available
Title
The phagocytosis of gas-filled microbubbles by human and murine antigen-presenting cells
Abstract

This study was designed to evaluate the potential of gas-filled microbubbles (MB) to be internalized by antigen-presenting cells (APC). Fluorescently labeled MB were prepared, thus permitting to track binding to, and internalization in, APC. Both human and mouse cells, including monocytes and dendritic cells (DC), prove capable to phagocyte MB in vitro. Observation by confocal laser scanning microscopy showed that interaction between MB and target cells resulted in a rapid internalization in cellular compartments and to a lesser extent in the cytoplasm. Capture of MB by APC resulted in phagolysosomal targeting as verified by double staining with anti-lysosome-associated membrane protein-1 monoclonal antibody and decrease of internalization by phagocytosis inhibitors. Fluorescent MB injected subcutaneously (s.c.) in mice were found to be associated with CD11c+DC in lymph nodes draining the injection sites 24 h after administration. Altogether, our study demonstrates that MB can successfully target APC both in vitro and in vivo, and thus may serve as a potent Ag delivery system without requirement for ultrasound-based sonoporation. This adds to the potential of applications of MB already extensively used for diagnostic imaging in humans.

Keywords
Microparticles; Gas-filled microbubbles; Antigen-presenting cells; Dendritic cells; Cellular uptakeMB, gas-filled microbubbles; P-MB, plain microbubbles; OG-MB, Oregon Green-labeled microbubbles; OG-DHPE, Oregon Green-dihexadecanoylphosphatidylethanolamin
First Page Preview
The phagocytosis of gas-filled microbubbles by human and murine antigen-presenting cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 1, January 2012, Pages 333–342
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering