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The generation of iPS cells using non-viral magnetic nanoparticlebased transfection

Paper ID Volume ID Publish Year Pages File Format Full-Text
7787 564 2011 9 PDF Available
Title
The generation of iPS cells using non-viral magnetic nanoparticlebased transfection
Abstract

Induced pluripotent stem (iPS) cells have been generated from various somatic cells; however, a major restriction of the technology is the use of potentially harmful genome-integrating viral DNAs. Here, without a viral vector, we generated iPS cells from fibroblasts using a non-viral magnetic nanoparticle-based transfection method that employs biodegradable cationic polymer PEI-coated super paramagnetic nanoparticles (NP). Our findings support the possible use of transient expression of iPS genes in somatic cells by magnet-based nanofection for efficient generation of iPS cells. Results of dynamic light scattering (DLS) analysis and TEM analyses demonstrated efficient conjugation of NP with iPS genes. After transfection, nanofection-mediated iPS cells showed ES cell-like characteristics, including expression of endogenous pluripotency genes, differentiation of three germ layer lineages, and formation of teratomas. Our results demonstrate that magnet-based nanofection may provide a safe method for use in generation of virus-free and exogenous DNA-free iPS cells, which will be crucial for future clinical applications in the field of regenerative medicine.

► We conduct a non-viral magnetic nanoparticle-based transfection to induce iPS from MEF. ► The magnetic nanoparticles are conjugated with 4 different iPS genes. ► The magnetic nanoparticle-based transfection of iPS genes provides safe and efficient generation of iPS.

Keywords
Induced pluripotent stem cells; Super paramagnetic nanoparticles; Non-viral transfection; Embryonic stem cells
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The generation of iPS cells using non-viral magnetic nanoparticlebased transfection
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 28, October 2011, Pages 6683–6691
Authors
, , , , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us