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Irreversible optical clearing of rabbit dermis for autogenic corneal stroma transplantation

Paper ID Volume ID Publish Year Pages File Format Full-Text
7795 564 2011 9 PDF Available
Title
Irreversible optical clearing of rabbit dermis for autogenic corneal stroma transplantation
Abstract

Tissue engineering and transplantation of autogenic grafts have been widely investigated for solving problems on current allograft treatments (i.g., donor shortage and rejection). However, it is difficult to obtain an autogenic corneal stromal replacement that is composed of transparent, tough, and thick collagen constructs by current cell culture-based tissue engineering. Aim of this study is to develop transparent dermis for an autogenic corneal stroma transplantation. This study examined dehydration at 4–8 °C and carbodiimide cross-linking on cloudy rabbit dermis (approx. 1.8%–3.8% light transmittance at 550 nm) for dermis optical clearing. Transparency of dehydrated rabbit dermis was founded to be approx. 37.9%–41.4% at 550 nm. Additional cross-linking treatment on dehydrated dermis prevented from swelling and clouding in saline, and improved its transparency to be 56.9% at 550 nm. Rabbit corneal epithelium was found to regenerate on optically cleared dermis in vitro. Furthermore, no abnormal biological response (i.e., inflammation, vascularization, and the barrier defect of epithelia) or no optical functional change on optically cleared dermis was observed during its 4-week autogenic transplantation into rabbit corneal stromal pocket.

Keywords
Autogenic transplantation; Corneal stroma; Dermis; Optical clearing; Chemical cross-linking
First Page Preview
Irreversible optical clearing of rabbit dermis for autogenic corneal stroma transplantation
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 28, October 2011, Pages 6764–6772
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering