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Affinity hydrogels for controlled protein release using nucleic acid aptamers and complementary oligonucleotides

Paper ID Volume ID Publish Year Pages File Format Full-Text
7803 564 2011 11 PDF Available
Title
Affinity hydrogels for controlled protein release using nucleic acid aptamers and complementary oligonucleotides
Abstract

Biomaterials for the precise control of protein release are important to the development of new strategies for treating human diseases. This study aimed to fundamentally understand aptamer–protein dissociation triggered by complementary oligonucleotides, and to apply this understanding to develop affinity hydrogels for controlled protein release. The results showed that the oligonucleotide tails of the aptamers played a critical role in inducing intermolecular hybridization and triggering aptamer–protein dissociation. In addition, the attachment of the oligonucleotide tails to the aptamers and the increase of hybridizing length could produce a synergistic effect on the dissociation of bound proteins from their aptamers. More importantly, pegylated complementary oligonucleotides could successfully trigger protein release from the aptamer-functionalized hydrogels at multiple time points. Based on these results, it is believed that aptamer-functionalized hydrogels and complementary oligonucleotides hold great potential of controlling the release of protein drugs to treat human diseases.

► We studied the effects of molecular hybridization on protein-aptamer dissociation. ► Both oligonucleotide tail and hybridizing length are important factors. ► Importantly, pegylated complementary oligonucleotides can trigger protein release. ► In addition, the triggered release can be achieved at multiple time points.

Keywords
Hydrogel; Affinity; Growth factor; Drug delivery; Controlled drug release
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Affinity hydrogels for controlled protein release using nucleic acid aptamers and complementary oligonucleotides
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 28, October 2011, Pages 6839–6849
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us