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pH-responsive polymers for trehalose loading and desiccation protection of human red blood cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
7820 565 2011 7 PDF Available
Title
pH-responsive polymers for trehalose loading and desiccation protection of human red blood cells
Abstract

PP-50, a synthetic pH-responsive biopolymer, is here shown to increase the permeability of the phospholipid bilayer to trehalose, a disaccharide accumulated in desiccation tolerant organisms across all kingdoms. Uptake of 251 ± 6 mm intracellular trehalose facilitated an increase in the membrane integrity of vacuum dried cells by a factor of 9 ± 1 and reduced extent of hemoglobin oxidation in dried cells from 66 ± 1% to 23 ± 3%. To elucidate the mechanism of PP-50 mediated trehalose delivery, permeability studies were conducted using molecules ranging in size from sucrose to 10 kDa poly(ethylene glycol). It was shown that the logarithm of relative diffusant membrane permeability decreased linearly with diffusant molecular volume, suggesting transport via non-Stokesian diffusion. Consistent with this conclusion, topographic atomic force micrographs reported membrane thinning proximate to PP-50 adsorption on the erythrocyte membrane, a phenomenon associated with increased incidence of phospholipid hydrocarbon chain bending.

Keywords
Biopolymer; Blood; Cryopreservation; Trehalose; Cell viability
First Page Preview
pH-responsive polymers for trehalose loading and desiccation protection of human red blood cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 19, July 2011, Pages 4443–4449
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering