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Comb-type grafted poly(N-isopropylacrylamide) gel modified surfaces for rapid detachment of cell sheet

Paper ID Volume ID Publish Year Pages File Format Full-Text
7850 567 2010 9 PDF Available
Title
Comb-type grafted poly(N-isopropylacrylamide) gel modified surfaces for rapid detachment of cell sheet
Abstract

A comb-type grafted poly(N-isopropylacrylamide) (PIPAAm) gel modified surface was newly developed for providing a rapid cell sheet recovery for tissue engineering. PIPAAm macromonomer was prepared by the etherification reaction of the hydroxyl terminal moieties of PIPAAm with acryloyl chloride, followed by the radical telomerization reaction of N-isopropylacrylamide (IPAAm) monomer using 2-mercaptoethanol as a chain transfer agent. Solution containing IPAAm monomer and PIPAAm macromonomer was spread on the surface of tissue culture polystyrene (TCPS), and then the surface was subjected to electron beam irradiation for grafting the monomer and macromonomer on the surfaces, resulting in comb-type grafted PIPAAm gel modified TCPS (GG-TCPS). Besides the difference of the amount of the modified PIPAAm, no distinct difference was found between the properties of GG-TCPSs and normal-type PIPAAm gel modified TCPS (NG-TCPS) through XPS, AFM and a contact angle measurement. At 37 °C, bovine aortic endothelial cells (BAECs) were well adhered and spread on GG-TCPS as well as NG-TCPS regardless of the macromonomer concentration. By lowering temperature to 20 °C, BAECs detached themselves more rapidly from GG-TCPS compared with NG-TCPS. Upon lowering temperature, the grafted polymer was speculated to accelerate the hydration of modified PIPAAm gel, resulting in a rapid cell sheet detachment.

Keywords
Macromonomer; Comb-type grafted poly(N-isopropylacrylamide) gel; Temperature-responsive cell culture surface; Cell sheet; Tissue engineering
First Page Preview
Comb-type grafted poly(N-isopropylacrylamide) gel modified surfaces for rapid detachment of cell sheet
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 29, October 2010, Pages 7435–7443
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering