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Rapid construction of mechanically- confined multi- cellular structures using dendrimeric intercellular linker

Paper ID Volume ID Publish Year Pages File Format Full-Text
7852 567 2010 13 PDF Available
Title
Rapid construction of mechanically- confined multi- cellular structures using dendrimeric intercellular linker
Abstract

Tissue constructs that mimic the in vivo cell–cell and cell–matrix interactions are especially useful for applications involving the cell- dense and matrix- poor internal organs. Rapid and precise arrangement of cells into functional tissue constructs remains a challenge in tissue engineering. We demonstrate rapid assembly of C3A cells into multi- cell structures using a dendrimeric intercellular linker. The linker is composed of oleyl- polyethylene glycol (PEG) derivatives conjugated to a 16 arms- polypropylenimine hexadecaamine (DAB) dendrimer. The positively charged multivalent dendrimer concentrates the linker onto the negatively charged cell surface to facilitate efficient insertion of the hydrophobic oleyl groups into the cellular membrane. Bringing linker- treated cells into close proximity to each other via mechanical means such as centrifugation and micromanipulation enables their rapid assembly into multi- cellular structures within minutes. The cells exhibit high levels of viability, proliferation, three- dimensional (3D) cell morphology and other functions in the constructs. We constructed defined multi- cellular structures such as rings, sheets or branching rods that can serve as potential tissue building blocks to be further assembled into complex 3D tissue constructs for biomedical applications.

Keywords
Rapid dendrimer; Oleyl poly (ethylene glycol) (oleyl-PEG); Cell- ECM interaction; Multicellular structures; Defined cellular structures; Tissue engineering
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Rapid construction of mechanically- confined multi- cellular structures using dendrimeric intercellular linker
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 29, October 2010, Pages 7455–7467
Authors
, , , , , , , , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us