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The impact of dendrimer-grafted modifications to model silicon surfaces on protein adsorption and bacterial adhesion

Paper ID Volume ID Publish Year Pages File Format Full-Text
7870 568 2011 12 PDF Available
Title
The impact of dendrimer-grafted modifications to model silicon surfaces on protein adsorption and bacterial adhesion
Abstract

In the oral cavity, omnipresent salivary protein films (pellicle) mediate bacterial adhesion and biofilm formation on natural tissues as well as on artificial implant surfaces, which may cause serious infectious diseases like periimplantitis. The purpose of this in vitro study was to investigate the adsorption/desorption behaviour of human saliva on model surfaces grafted with polyamidoamine (PAMAM) dendrimer molecules compared to self-assembled monolayers (SAMs) exhibiting the same terminal functions (–NH2, –COOH) by two complementary analytical methods. Furthermore, the role of saliva conditioning of PAMAM and analogous SAM modifications on the adhesion of Streptococcus gordonii DL1, an early oral colonizer, was investigated. In contrast to SAMs, PAMAM-grafted surfaces showed reduced streptococcal adherence in the absence of pre-adsorbed saliva similar to the level obtained for poly(ethylene glycol) (PEG) coatings. Moreover, coatings of PAMAM-NH2 maintained their bacteria-repellent behaviour even after saliva-conditioning. As a general outcome, it was found that lower amounts of protein adsorbed on PAMAM coatings than on analogous SAMs. Since this study demonstrates that covalently bound PAMAM dendrimers can modulate the oral bacterial response, this approach has significant potential for the development of anti-adhesive biomaterial surfaces that are conditioned with proteinaceous films.

Keywords
Polyamidoamine (PAMAM) dendrimer; Saliva conditioning film; Quartz crystal microbalance (QCM-D); Bacterial adhesion; Streptococcus gordonii DL1; Dental implant
First Page Preview
The impact of dendrimer-grafted modifications to model silicon surfaces on protein adsorption and bacterial adhesion
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 35, December 2011, Pages 9168–9179
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering