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Long-term cytokine-free expansion of cord blood mononuclear cells in three-dimensional scaffolds

Paper ID Volume ID Publish Year Pages File Format Full-Text
7879 568 2011 8 PDF Available
Title
Long-term cytokine-free expansion of cord blood mononuclear cells in three-dimensional scaffolds
Abstract

Cord blood expansion ex vivo can be achieved in liquid suspension through the addition of cytokines at the expense of often undesirable cell differentiation. In order to derive a cytokine-free dynamic culture system, we hypothesised that a three-dimensional (3D) environment in the form of highly porous scaffolds made of poly (d,l-lactide-co-glycolide) (PLGA) or polyurethane (PU) for the biomimetic growth of cord blood mononuclear cells (CBMNCs), would facilitate expansion of hematopoietic cells without exogenous cytokines. Both scaffolds supported cellular expansion ex vivo. Cytokine-free, long-term culture was best in PU coated with collagen type I (54-fold expansion). In contrast, traditional 2D well-plate cultures collapsed within 4 days in the absence of cytokines. CBMNCs cultured in the scaffolds were visualised by scanning electron microscopy and immunophenotypic/immunostaining analysis and the studies validated the presence of a dynamic culture containing erythroid precursors (CD45−/CD71+/CD235a+), hematopoietic stem/progenitor cells (CD38−CD34+, CD117+), maturing myeloid cells (CD38+, MPO+), CD4+ and CD8+ T-lymphocytes and megakaryocytes (FVIII+). Colony forming unit (CFU) assays indicated that BFU-E and CFU-GM increased (p < 0.05) whereas CFU-GEMM were maintained at week 4. In conclusion, this 3D culture system is capable of long-term, cytokine-free expansion of CBMNCs, enabling the study of hematopoiesis and providing a potential platform for drug discovery and therapeutic applications ex vivo.

Keywords
Biomimetic biomaterials; Bone marrow tissue engineering; Cytokine-free; Cord blood stem cells
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Long-term cytokine-free expansion of cord blood mononuclear cells in three-dimensional scaffolds
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 35, December 2011, Pages 9263–9270
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us