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The promotion of microvasculature formation in poly(ethylene glycol) diacrylate hydrogels by an immobilized VEGF-mimetic peptide

Paper ID Volume ID Publish Year Pages File Format Full-Text
7912 569 2011 8 PDF Available
Title
The promotion of microvasculature formation in poly(ethylene glycol) diacrylate hydrogels by an immobilized VEGF-mimetic peptide
Abstract

Microvascularization of tissue engineered constructs was achieved by utilizing a VEGF-mimicking peptide, QK, covalently bound to a poly(ethylene glycol) hydrogel matrix. The 15-amino acid peptide, developed by D’Andrea et al., was modified with a PEG-succinimidyl ester linker on the N-terminus of the peptide, then photocrosslinked onto the surface or throughout PEG hydrogels. PEGylation of the peptide increased its solubility and bioactivity, as evidenced by endothelial cell proliferation. PEG-QK showed equal or superior ability to promote angiogenesis in vitro, on the surface of hydrogels and within three-dimensional collagenase-degradable hydrogels, compared to RGDS only or PEG-VEGF hydrogels. Endothelial cells were shown to form tubule structures, migrate, and make cell–cell contacts in response to covalently-bound PEG-QK. In vivo in a mouse cornea micropocket angiogenesis assay, PEG-QK hydrogels promoted more complete coverage of host microvasculature within the hydrogel. PEG-QK was shown to enhance vessel branch points and vessel density as well as space filling properties of fractal dimension and lacunarity. This report shows the ability to promote angiogenesis in tissue engineered constructs using a covalently-bound small peptide rather than a large protein and may point to an advance in designing biomimetic cellular environments.

Keywords
Angiogenesis; Peptide; Poly(ethylene glycol); Vascularization
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The promotion of microvasculature formation in poly(ethylene glycol) diacrylate hydrogels by an immobilized VEGF-mimetic peptide
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 25, September 2011, Pages 5782–5789
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us