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Non-destructive label-free monitoring of collagen gel remodeling using optical coherence tomography

Paper ID Volume ID Publish Year Pages File Format Full-Text
7941 570 2010 8 PDF Available
Title
Non-destructive label-free monitoring of collagen gel remodeling using optical coherence tomography
Abstract

Matrix remodeling plays a fundamental role in physiological and pathological processes, as well as in tissue engineering applications. In this paper, optical coherence tomography (OCT), a non-destructive optical imaging technology, was used to image collagen gel remodeling by smooth muscle cells (SMCs). The optical scattering properties of collagen–SMC gels were characterized quantitatively by fitting OCT data to a theoretical model. Matrix remodeling over 5 days produced a 10-fold increase in the reflectivity of the collagen gels, corresponding to a decrease in scattering anisotropy from 0.91 to 0.46. The increase in reflectivity was corroborated in confocal mosaic images. Blocking matrix degradation in collagen–SMC gels with doxycycline, a non-specific matrix metalloproteinases (MMPs) inhibitor, impeded the decrease in scattering anisotropy and resulted in few macroscopic signs of remodeling. Causing matrix degradation in acellular gels with a 3 h treatment of MMP-8 (collagenase 2) partially mimicked the decrease in anisotropy measured in collagen–SMC gels after 5 days. These results suggest that the decrease in scattering anisotropy in the collagen–SMC gels was due to MMP activity that degrades collagen fibrils into smaller fragments.

Keywords
ECM (extracellular matrix); Collagen; Smooth muscle cell; Matrix metalloproteinases; Optical coherence tomography
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 32, November 2010, Pages 8210–8217
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us