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Patterning network structure to spatially control cellular remodeling and stem cell fate within 3-dimensional hydrogels

Paper ID Volume ID Publish Year Pages File Format Full-Text
7943 570 2010 7 PDF Available
Title
Patterning network structure to spatially control cellular remodeling and stem cell fate within 3-dimensional hydrogels
Abstract

The spatially directed 3-dimensional (3D) remodeling of synthetic materials may be useful to regionally control cell behavior. In this work, we developed a process to synthesize hyaluronic acid hydrogels using multiple modes of crosslinking applied sequentially; a primary addition reaction to introduce protease degradable peptide crosslinks, then a UV light-induced secondary radical reaction (enabling spatial control) to introduce non-degradable kinetic chains. These differential network structures either permitted (primary crosslinking only, “−UV”) or inhibited (sequential crosslinking, “+UV”) cellular remodeling. This behavior was validated by controlling the outgrowth from chick aortic arches or the spreading of encapsulated mesenchymal stem cells (MSCs), where only −UV regions permitted arch outgrowth and MSC spreading. Additionally, network structures dictated adipogenic/osteogenic MSC fate decisions, with spatial control, by controlling encapsulated MSC spreading. This manipulation of microenvironmental cues may be valuable for advanced tissue engineering applications requiring the spatial control of cells in 3D.

Keywords
Hydrogels; Stem cells; Patterning; Remodeling; Hyaluronic acid
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Patterning network structure to spatially control cellular remodeling and stem cell fate within 3-dimensional hydrogels
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 32, November 2010, Pages 8228–8234
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us