fulltext.study @t Gmail

Perfusion and characterization of an endothelial cell-seeded modular tissue engineered construct formed in a microfluidic remodeling chamber

Paper ID Volume ID Publish Year Pages File Format Full-Text
7946 570 2010 8 PDF Available
Title
Perfusion and characterization of an endothelial cell-seeded modular tissue engineered construct formed in a microfluidic remodeling chamber
Abstract

Tissue engineered constructs containing tortuous endothelial cell-lined perfusion channels were formed by randomly assembling endothelial cell-seeded submillimeter-sized collagen cylinders (modules) into a microfluidic perfusion chamber. The interconnected void space produced by random module packing created flow channels that were lined with endothelial cells. The effect of perfusion (0.5 mL min−1, Re∗ = 27.78 and shear stress = 0.16 dyn cm−2) through the tortuous channels on construct remodeling and endothelium quiescence was studied. Over time, modules fused at their points of contact and as they contracted, decreased the internal void space, which reduced the overall perfusion through the construct. As compared to static controls, perfusion caused a transient increase in activation (ICAM-1 and VCAM-1 expression) after 1 h followed by a decrease after 24 h. Proliferation (by BrdU) was reduced significantly, while KLF2, which is upregulated with atheroprotective laminar shear stress, was upregulated significantly after 24 h. VE-cadherin became discontinuous and was significantly downregulated after 24 h, which was likely caused by the dismantling of the endothelial cell adherens junctions during remodeling. Collectively, these outcomes suggest that flow through the construct did not drive the endothelial cells towards an inflamed, “atherosclerotic like” disturbed flow pathology.

Keywords
Tissue engineering; Perfusion; Endothelial cells; Activation; VE-cadherin, KLF2, ICAM-1 and VCAM-1; Collagen
First Page Preview
Perfusion and characterization of an endothelial cell-seeded modular tissue engineered construct formed in a microfluidic remodeling chamber
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 32, November 2010, Pages 8254–8261
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us