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A polymer microstructure array for the formation, culturing, and high throughput drug screening of breast cancer spheroids

Paper ID Volume ID Publish Year Pages File Format Full-Text
7965 570 2010 9 PDF Available
Title
A polymer microstructure array for the formation, culturing, and high throughput drug screening of breast cancer spheroids
Abstract

Multicellular spheroid models have been recognized as superior to monolayer cell cultures in antitumor drug screening, but their commercial adaptation in the pharmaceutical industry has been delayed, primarily due to technological limitations. The current study presents a new spheroid culture platform that addresses these technical restrictions. The new culturing device is based on a multiwell plate equipped with a glass bottom patterned with an array of UV adhesive microchambers. Each microchamber is designed to accommodate a single spheroid. The system facilitates the simultaneous creation and culturing of a large number of spheroids, as well as screening their response to antitumor drugs. The volume of the spheroids is easily controlled by seeding density. The location of each spheroid is preserved in the same microchamber throughout its growth, treatment with soluble agents, and imaging. The growth ratio parameter, a non-intrusive size analysis of the same spheroid before and after exposure to drugs, was found to be a sensitive indicator for the reaction of MCF7 breast cancer spheroids to cytotoxic drugs. This feature helps reveal the heterogeneity within the spheroid population during the formation process and their drug response, and provides an opportunity to detect specific, highly active or drug-resistant spheroid sub-groups. The advantages of this spheroid-based system make it an efficient drug-screening tool that may be valuable to related fields of research and clinical applications.

Keywords
Spheroid-based drug screening; 3D culturing; Multicellular breast cancer spheroids; Microchamber array; UV adhesive microstructure
First Page Preview
A polymer microstructure array for the formation, culturing, and high throughput drug screening of breast cancer spheroids
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 32, November 2010, Pages 8436–8444
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering