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A cell sorter with modified bamboo charcoal for the efficient selection of specific antibody-producing hybridomas

Paper ID Volume ID Publish Year Pages File Format Full-Text
7966 570 2010 9 PDF Available
Title
A cell sorter with modified bamboo charcoal for the efficient selection of specific antibody-producing hybridomas
Abstract

Monoclonal antibodies (mAbs) have been proven useful in research and clinical applications. However, the generation of mAbs by conventional hybridoma technology is time-, cost- and labor-consuming. Here we developed a simplified procedure for efficient generation and selection of antibody-producing hybridomas within 1 h, using a particular cell sorter design, a cytoflow reactor-based cell sorter (CBCS) which consists mainly of the “cytoflow reactor” that comprises two components, a reaction chamber and a glass tubing for air and medium exchange by gravity, and the “sorting material”, human EGFR-conjugated bamboo charcoal, for specific B-cell enrichment. The high surface area and porous structure of bamboo charcoal greatly increased cell density and protein production. Moreover, from Raman, FT-IR spectroscopy and IFA analysis, the carboxylation and immobilization of bamboo charcoal can be introduced easily by nitric acid treatment and conjugated handily with human EGFR using EDC/NHS. Other evidences, such as IFA, showed that the specific hybridomas generated in this study could secrete specific anti-human EGFR antibodies. Our design allows the production of mAbs while avoiding time-consuming steps, such as large numbers of limiting dilutions and screening assays, and demonstrates that the CBCS could be a powerful tool for monoclonal antibody production.

Keywords
Bioreactor; Hybridomas; Cytoflow reactor; Monoclonal antibody; Bamboo charcoal; Surface modification
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A cell sorter with modified bamboo charcoal for the efficient selection of specific antibody-producing hybridomas
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 32, November 2010, Pages 8445–8453
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us