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The three-dimensional vascularization of growth factor-releasing hybrid scaffold of poly (ɛ-caprolactone)/collagen fibers and hyaluronic acid hydrogel

Paper ID Volume ID Publish Year Pages File Format Full-Text
8000 573 2011 10 PDF Available
Title
The three-dimensional vascularization of growth factor-releasing hybrid scaffold of poly (ɛ-caprolactone)/collagen fibers and hyaluronic acid hydrogel
Abstract

A significant stumbling block in the creation of functional three-dimensional (3D) engineered tissues is the proper vascularization of the constructs. Furthermore, in the context of electrospinning, the development of 3D constructs using this technique has been hindered by the limited infiltration of cells into their structure. In an attempt to address these issues, a hybrid mesh of poly (ɛ-caprolactone)-collagen blend (PCL/Col) and hyaluronic acid (HA) hydrogel, Heprasil™ was created via a dual electrodeposition system. Simultaneous deposition of HA and PCL/Col allowed the dual loading and controlled release of two potent angiogenic growth factors VEGF165 and PDGF-BB over a period of five weeks in vitro. Furthermore, this manner of loading sustained the bioactivity of the two growth factors. Utilizing an in-house developed 3D co-culture assay model of human umbilical vein endothelial cells and lung fibroblasts, the growth factor-loaded hybrid meshes was shown to not only support cellular attachment, but also their infiltration and the recapitulation of primitive capillary network in the scaffold’s architecture. Thus, the creation of a PCL/Col-Heprasil hybrid scaffold is a step forward toward the attainment of a 3D bio-functionalized, vascularized tissue engineering construct.

Keywords
Angiogenesis; Hyaluronic acid/hyaluronan; Polycaprolactone; Collagen; Scaffold; Endothelial cell
First Page Preview
The three-dimensional vascularization of growth factor-releasing hybrid scaffold of poly (ɛ-caprolactone)/collagen fibers and hyaluronic acid hydrogel
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 32, November 2011, Pages 8108–8117
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering