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Creation of a blood-compatible surface: A novel strategy for suppressing blood activation and coagulation using a nitroxide radical-containing polymer with reactive oxygen species scavenging activity

Paper ID Volume ID Publish Year Pages File Format Full-Text
803 63 2012 7 PDF Available
Title
Creation of a blood-compatible surface: A novel strategy for suppressing blood activation and coagulation using a nitroxide radical-containing polymer with reactive oxygen species scavenging activity
Abstract

Various polymeric materials have been used in medical devices, including blood-contacting artificial organs. Contact between blood and foreign materials causes blood cell activation and adhesion, followed by blood coagulation. Concurrently, the activated blood cells release inflammatory cytokines together with reactive oxygen species (ROS). We have hypothesized that the suppression of ROS generation plays a crucial role in blood activation and coagulation. To confirm this hypothesis, surface-coated polymers containing nitroxide radical compounds (nitroxide radical-containing polymers (NRP)) were designed and developed. The NRP was composed of a hydrophobic poly(chloromethylstyrene) (PCMS) chain to which 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO) moieties were conjugated via condensation reaction of the chloromethyl groups in PCMS with the sodium alcoholate group of 4-hydroxy-TEMPO. Blood compatibility was investigated by placing NRP-coated beads in contact with rat whole blood. The amount of ROS generated on PCMS-coated beads used as a control increased significantly with time, while NRP-coated beads suppressed ROS generation. It is interesting to note that the suppression of inflammatory cytokine generation by NRP-coated beads was shown to be significantly higher than that by PCMS-coated beads. Both platelet and leukocyte adhesion to the beads were suppressed with increasing TEMPO incorporation in the polymer. These results confirm that the suppression of ROS by NRP prevents inflammatory cytokine generation, which in turn results in the suppression of blood activation and coagulation on the beads.

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Keywords
Reactive oxygen species; Inflammation; Anti-thrombogenic biointerface; Blood compatibility; Cell activation
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Creation of a blood-compatible surface: A novel strategy for suppressing blood activation and coagulation using a nitroxide radical-containing polymer with reactive oxygen species scavenging activity
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 8, Issue 3, March 2012, Pages 1323–1329
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us