fulltext.study @t Gmail

Cell transcytosing poly-arginine coated magnetic nanovector for safe and effective siRNA delivery

Paper ID Volume ID Publish Year Pages File Format Full-Text
8085 576 2011 9 PDF Available
Title
Cell transcytosing poly-arginine coated magnetic nanovector for safe and effective siRNA delivery
Abstract

Lack of safe and effective carriers for delivery of RNA therapeutics remains a barrier to its broad clinical application. We report the development of a cell tanscytosing magnetic nanovector engineered as an siRNA carrier. Iron oxide nanoparticles were modified with poly(ethylene glycol) (PEG), small interfering RNA (siRNA), and a cationic polymer layer. Three nanovector formulations with cationic polymer coatings of poly-arginine (pArg), polylysine (pLys), and polyethylenimine (PEI), respectively, were prepared. The three nanovector formulations where evaluated for safety and ability to promote gene silencing in three types of cancer cells C6/GFP+, MCF7/GFP+, and TC2/GFP+, mimicking human cancers of the brain, breast, and prostate, respectively. Cell viability and fluorescence quantification assays revealed that pArg-coated nanovectors were most effective in promoting gene knockdown and least toxic of the three nanovector formulations tested. Transmission electron microscopy (TEM) imaging of nanovector treated cells further demonstrated that pArg-coated nanovectors enter cells through cell transcytosis, while pLys and PEI coated nanovectors enter cells endocytosis. Our findings suggest that NPs engineered to exploit the cell transcytosis intracellular trafficking pathway may offer a more safe and efficient route for siRNA delivery.

Keywords
Iron oxide nanoparticle; Gene therapy; Transcytosis; Cancer; MRI; siRNA
First Page Preview
Cell transcytosing poly-arginine coated magnetic nanovector for safe and effective siRNA delivery
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 24, August 2011, Pages 5717–5725
Authors
, , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering