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Screening platelet–surface interactions using negative surface charge gradients

Paper ID Volume ID Publish Year Pages File Format Full-Text
8119 578 2010 8 PDF Available
Title
Screening platelet–surface interactions using negative surface charge gradients
Abstract

Negative surface charge density gradients were prepared on fused silica slides using selective oxidation of a 3-mercaptopropyltrimethoxysilane (MTS) monolayer converting surface thiol groups (–SH) into negatively charged sulfonate (−SO3−) groups. The sulfonate-to-thiol gradient samples were characterized by water contact angle and electron spectroscopy for chemical analysis (ESCA). Gradients were pre-adsorbed with proteins from three different solutions: platelet free plasma (PFP), fibrinogen, or albumin in phosphate buffered saline (PBS). Washed platelets were perfused over gradient samples in a parallel plate flow chamber and platelet adhesion was measured across the gradients using differential interference contrast (DIC) microscopy. Gradients pre-adsorbed with PFP showed adhesion contrast inversely related to the negative surface charge density. The magnitude of the adhesion contrast along the gradient was also dependent on PFP concentration. Gradients pre-adsorbed with fibrinogen showed an adhesion maximum in the center of the gradient region. Albumin coating of the gradients resulted in low overall platelet adhesion with increased adhesion in regions of high negative charge density. The effect of gradient orientation with respect to the flow was also investigated. Gradients pre-adsorbed with 10% PFP showed different adhesion contrast when the platelets were perfused in opposite directions. This suggests that platelet adhesion is, in addition to responding to the local surface properties, also dependent on the upstream conditions.

Keywords
Platelet adhesion; Surface gradient; Fibrinogen; Albumin; Plasma proteins; Blood compatibility
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 12, April 2010, Pages 3148–3155
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us