fulltext.study @t Gmail

Cell surface receptor-specific scaffold requirements for adhesion to laminin-derived peptide–chitosan membranes

Paper ID Volume ID Publish Year Pages File Format Full-Text
8128 578 2010 7 PDF Available
Title
Cell surface receptor-specific scaffold requirements for adhesion to laminin-derived peptide–chitosan membranes
Abstract

Scaffolds are used for bioengineering to regulate cellular functions. Previously, we developed laminin-derived peptide–conjugated chitosan membranes for cell engineering. Here, we determined whether changes in the chitosan scaffold altered the cellular response. When an αvβ3 integrin-binding peptide A99a (ALRGDN) was conjugated on chitosan membranes of varying density (1.5–1500 ng/mm2), cell adhesion was altered depending on the amount of chitosan. 3 or 30 ng/mm2 of the A99a-chitosan membrane effectively promoted cell attachment, cell spreading with well-organized actin stress fibers, phosphorylation of FAK Tyr397, and neurite outgrowth. In contrast, syndecan-binding peptide AG73 (RKRLQVQLSIRT) conjugated chitosan membranes density (1.5–1500 ng/mm2) promoted similar biological activities at all of the concentrations tested. These results suggest that integrin-mediated cell adhesion is sensitive to the scaffold condition. To improve the function of integrin-mediated biological activities on a large amount of scaffold, we designed an A99a/AG73 mixed peptide–chitosan membrane. The mixed peptide–chitosan membrane promoted the strongest biological activities at 150–1500 ng/mm2 of chitosan membrane. We conclude that the A99a/AG73 mixed peptide–chitosan membrane effectively interacts with both integrins and syndecans and is a useful multi-functional biomaterial.

Keywords
Scaffold; Peptide; Chitin/chitosan; Integrin; ECM (extracellular matrix); Cell adhesionECM, extracellular matrix; Fmoc, 9-fluorenylmethoxycarbonyl; DMF, dimethylformamide; FBS, fetal bovine serum; BSA, bovine serum albumin; HDFs, human dermal fibroblasts;
First Page Preview
Cell surface receptor-specific scaffold requirements for adhesion to laminin-derived peptide–chitosan membranes
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 12, April 2010, Pages 3237–3243
Authors
, , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us