fulltext.study @t Gmail

Cellular transduction gradients via vapor-deposited polymer coatings

Paper ID Volume ID Publish Year Pages File Format Full-Text
8155 579 2011 7 PDF Available
Title
Cellular transduction gradients via vapor-deposited polymer coatings
Abstract

Spatiotemporal control of gene delivery, particularly signaling gradients, via biomaterials poses significant challenges because of the lack of efficient delivery systems for therapeutic proteins and genes. This challenge was addressed by using chemical vapor deposition (CVD) polymerization in a counterflow set-up to deposit copolymers bearing two reactive chemical gradients. FTIR spectroscopy verified the formation of compositional gradients. Adenovirus expressing a reporter gene was biotinylated and immobilized using the VBABM method (virus-biotin-avidin-biotin-materials). Sandwich ELISA confirmed selective attachment of biotinylated adenovirus onto copolymer gradients. When cultured on the adenovirus gradients, human gingival fibroblasts exhibited asymmetric transduction with full confluency. Importantly, gradient transduction occurred in both lateral directions, thus enabling more advanced delivery studies that involve gradients of multiple therapeutic genes.

Keywords
Tissue engineering; Gene therapy; CVD coatings; Polymers; Virus immobilization
First Page Preview
Cellular transduction gradients via vapor-deposited polymer coatings
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 7, March 2011, Pages 1809–1815
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering