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Robust MeO2MA/vinyl-4,6-diamino-1,3,5-triazine copolymer hydrogels-mediated reverse gene transfection and thermo-induced cell detachment

Paper ID Volume ID Publish Year Pages File Format Full-Text
8169 579 2011 7 PDF Available
Title
Robust MeO2MA/vinyl-4,6-diamino-1,3,5-triazine copolymer hydrogels-mediated reverse gene transfection and thermo-induced cell detachment
Abstract

We have fabricated a robust temperature sensitive hydrogel by photoinitiated copolymerization of 2-(2-methoxyethoxy)ethyl methacrylate (MeO2MA), 2-vinyl-4,6-diamino-1,3,5-triazine (VDT) and crosslinker polyethylene glycol diacrylate (PEGDA). It was shown that self-hydrogen bondings of VDT moieties in the bulk considerably strengthened the mechanical properties of gels, which was dependent on the weight ratio of MeO2/VDT and the initial monomer concentration; the VDT motifs on the surface could efficiently bind DNA for reverse gene transfection. On this soft-wet platform, gene expression lasted 7 days and the re-treated gels could be reused for new cycle of transfection. The gene modified cells could be detached by thermo-triggered switchable surface hydrophilicity of PMEO2MA in hydrogel. MTT assay showed low cytotoxicity of hydrogels. The results suggested that this type of mechanically strong H-bonded and thermoresponsive hydrogels hold a great potential as an integrated functional soft-wet platform for the unharmful harvest of gene modified seed cells for tissue engineering or as implantable scaffold for gene therapy and regenerative medicine applications.

Keywords
2-(2-Methoxyethoxy)ethyl methacrylate; 2-Vinyl-4,6-diamino-1,3,5-triazine; Reverse transfection; Hydrogel; Cell detachment
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Robust MeO2MA/vinyl-4,6-diamino-1,3,5-triazine copolymer hydrogels-mediated reverse gene transfection and thermo-induced cell detachment
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 7, March 2011, Pages 1943–1949
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us