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Bioactivation of dermal scaffolds with a non-viral copolymer-protected gene vector

Paper ID Volume ID Publish Year Pages File Format Full-Text
8175 579 2003 8 PDF Available
Title
Bioactivation of dermal scaffolds with a non-viral copolymer-protected gene vector
Abstract

The use of scaffolds in skin tissue engineering is accompanied with low regeneration rates and high risk of infection. In this study, we activated an FDA-approved collagen scaffold for dermal regeneration by incorporation of copolymer-protected gene vectors (COPROGs) to induce a temporary release of VEGF. In vitro results show that the presence of COPROGs did not affect the distribution, attachment, proliferation and viability of cells in the scaffold. A transient release of VEGF was observed for up to 3 weeks. Moreover a high amount of VEGF was also found in the cells and associated with the scaffold. In a full skin defect model in nude mice, VEGF levels were significantly increased compared to controls in VEGF gene activated scaffolds 14 d after implantation, but not in skin from the wound edge. Results showed an increased amount of non-adherent cells, especially erythrocytes, and von Willebrandt factor (vWF) and a yellow red appearance of gene activated scaffolds in relation to controls. This suggests the presence of leaky vessels. In this work we show that the bioactivation of collagen scaffolds with COPROGs presents a new technology that allows a local release of therapeutic proteins thus enhancing the regenerative potential in vivo.

Keywords
Non-viral gene therapy; Gene activated matrix; Controlled drug release; Nanobiotechnology; Angiogenesis; Wound healing
First Page Preview
Bioactivation of dermal scaffolds with a non-viral copolymer-protected gene vector
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 7, March 2011, Pages 1996–2003
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering