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One-to-one quantum dot-labeled single long DNA probes

Paper ID Volume ID Publish Year Pages File Format Full-Text
8215 581 2011 7 PDF Available
Title
One-to-one quantum dot-labeled single long DNA probes
Abstract

Quantum dots (QDs) have been received most attention due to their unique properties. Constructing QDs conjugated with certain number of biomolecules is considered as one of the most important research goals in nanobiotechnology. In this study, we report polymerase chain reaction (PCR) amplification of primer oligonucleotides bound to QDs, termed as QD-based PCR. Characterization of QD-based PCR products by gel electrophoresis and atomic force microscopy showed that QD-labeled long DNA strands were synthesized and only a single long DNA strand was conjugated with a QD. The QD-based PCR products still kept fluorescence properties. Moreover, the one-to-one QD-labeled long DNA conjugates as probes could detect a single-copy gene on maize chromosomes by fluorescence in situ hybridization. Labeling a single QD to a single long DNA will make detection of small single-copy DNA fragments, quantitative detection and single molecule imaging come true by nanotechnology, and it will promote medical diagnosis and basic biological research as well as nano-material fabrication.

Keywords
Nanoparticle; DNA; PCR (polymerase chain reaction); In situ hybridization
First Page Preview
One-to-one quantum dot-labeled single long DNA probes
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 23, August 2011, Pages 5471–5477
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering